Family: Francesco I Grand Duke of Tuscany De Medici / Joanna Archduchess of Austria (F39953)
m. 18 Dec 1565
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 | Cosimo I (Grand Duke of Tuscany) De Medici (1519-1574)
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 | Lucrezia De Medici (1544- ) |
| Ferdinando I (Grand Duke of Tuscany) De Medici (1549-1609)  |
| Ferdinand I Habsburg (King of Hungary) of Austri (1503-1564)
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| Anne of Bohemia (1503-1547)
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| Maximilian II Hapsburg Holy Roman Emperor (1527-1576) |
| Anna (of Austria) Habsburg (1528-1590) |
| Marie Archduchess of Austria (1531-1581) |
| Charles Habsburg Archduke of Austria (1540-1590) |
| Francesco I Grand Duke of Tuscany De Medici (1541-1587)
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| Joanna Archduchess of Austria (1546-1578)
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| Marie De Medici (1573-1642) |
| Eleonora De Medici (1566-1611) |