Family: James (4th Earl of Bothwell) Hepburn / Mary Queen of Scots (F39649)
m. 15 May 1567
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 | Patrick (3rd Earl of Bothwell) Hepburn (1512-1556)
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 | Agnes Sinclair ( -1574)
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| Jane Hepburn ( -1599)  |
| James V Stewart King of Scotland (1512-1542)
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| Mary De Guise De Lorraine (1515-1560)
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| James Stuart (1540-1541) |
| Arthur Stuart (1541-1541) |
| Mary Queen of Scots (1542-1587)
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| James (4th Earl of Bothwell) Hepburn (1536-1578)
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| Twin Hepburn (1568-1568) |